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|Title:||Investigations and Applications of Metal Ion Enhanced Chemiluminescence|
|Department / Program:||Chemistry|
|Degree Granting Institution:||University of Illinois at Urbana-Champaign|
|Abstract:||Development of an HPLC detector for amino acids based on the suppression of the Co(II) enhanced luminol reaction is presented in this work. Compared to the classical ninhydrin reaction, this method is faster, can be run at room temperature without thermostatting, can determine secondary amino acids without varying the reaction or instrument, has simpler instrumentation, and, in favorable cases, has lower detection limits. The data suggest that the mechanism for inhibition is complexation of Co(II) by the amino acid.
Also presented in this work are data relating to attempt to obtain selectivity for various metal ions using chemiluminescent detection. The effects of varying reagent concentrations on the chemiluminescent enhancement of various metal ions of the luminol, lophine and gallic acid reactions are shown. These data reveal a complexity which was unexpected and leads to the conclusion that more fundamental work on the role of the metal ion in chemiluminescent reactions is needed before attempts at specificity can be made.
Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 1984.
|Date Available in IDEALS:||2014-12-15|