Files in this item



application/pdf8711769.pdf (3MB)Restricted to U of Illinois
(no description provided)PDF


Title:Molecular Biology of the Cytochrome O Terminal Oxidase of Escherichia Coli (Cloning, Sequencing, Dna)
Author(s):Au, Chi-Tak Doug
Department / Program:Chemistry
Degree Granting Institution:University of Illinois at Urbana-Champaign
Subject(s):Chemistry, Biochemistry
Abstract:My thesis research has dealt mainly with a genetic approach to studying the cytochrome o terminal oxidase complex of Escherichia coli. Respiratory-deficient mutants of E. coli have been isolated which are unable to grow aerobically on non-fermentable substrates such as succinate and lactate. Spectroscopic and immunological studies showed that these mutants lack the cytochrome o terminal oxidase of the high aeration branch of the respiratory chain. These strains carry mutations in a locus designated cyo which maps at min 10.2 on the E. coli linkage map. The structural genes encoding the cytochrome o oxidase complex have been cloned into the multicopy plasmid pBR322. Strains carrying the cyo plasmids produce 5 to 10 times more cytochrome o oxidase than control strains. The gene products encoded by the cyo plasmids can be immunoprecipitated with monospecific antibodies raised against cytochrome o. The cyo gene locus is located in a 5.5-kb chromosomal DNA segment. The nucleotide sequence of a portion of the cyo gene locus has been determined by Sanger's dideoxynucleotide sequencing method. Further genetic manipulations and a complete DNA sequence analysis of the cyo gene locus will provide valuable information on the protein structure-function relationships.
Issue Date:1987
Description:103 p.
Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 1987.
Other Identifier(s):(UMI)AAI8711769
Date Available in IDEALS:2014-12-15
Date Deposited:1987

This item appears in the following Collection(s)

Item Statistics