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Title:The Phosphatase Activities of T4 Polynucleotide Kinase (Rna Ligase, Splicing)
Author(s):Weber, Thomas Richard
Department / Program:Biochemistry
Discipline:Biochemistry
Degree Granting Institution:University of Illinois at Urbana-Champaign
Degree:Ph.D.
Genre:Dissertation
Subject(s):Chemistry, Biochemistry
Abstract:The recent elucidation of the role of the 2':3'-cyclic phosphate in the mechanism of tRNA splicing in wheat germ and yeast prompted a closer examination of the 3'-phosphatase activity of T4 polynucleotide kinase. This resulted in the discovery of not only a 2':3'-cyclic phosphatase activity but also a 2'-phosphatase, in addition to the previously demonstrated 3'-phosphatase. The relationship between the three activities is characterized by determining the pH rate profile, kinetic constants for both ribonucleotide and deoxyribonucleotide substrates and length dependence of each activity. The data suggests that all three activities occur at the same active site. In addition, a model is proposed in which the 2':3'-cyclic phosphatase and kinase activities function with RNA ligase in tRNA breakage and religation.
Issue Date:1985
Type:Text
Description:116 p.
Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 1985.
URI:http://hdl.handle.net/2142/70541
Other Identifier(s):(UMI)AAI8511690
Date Available in IDEALS:2014-12-15
Date Deposited:1985


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