Files in this item

FilesDescriptionFormat

application/pdf

application/pdf8303004.pdf (3MB)Restricted to U of Illinois
(no description provided)PDF

Description

Title:Novel Compounds From Methanogens: Characterization of Component B of the Methylreductase System and Mobile Factor
Author(s):Tanner, Ralph S.
Department / Program:Microbiology
Discipline:Microbiology
Degree Granting Institution:University of Illinois at Urbana-Champaign
Degree:Ph.D.
Genre:Dissertation
Subject(s):Biology, Microbiology
Abstract:An enzymic assay for the detection and quantitation of component B based on cell-free extract of Methanobacterium thermoautotrophicum stripped of small molecular weight cofactors by anaerobic column chromatography was developed. Component B was purified to homogeneity from boiled cell extract of M. thermoautotrophicum by anaerobic chromatography on Sephadex DEAE-A25, Sephadex G10, and Sephadex G25-F columns. Component B was determined to have a molecular weight of 1,044 by fast atom bombardment mass spectrometry, and a molecular formula of C(,32)H(,47)N(,3)O(,26)P(,2)Na(,4) was proposed for the sodium salt of the compound. A primary amine was present in component B, but no amino acid was detected. Component B was determined to have a monoester phosphate and a diester phosphate, and one mol of aldopentose per mol of component B. The UV absorbance spectrum revealed the presence of two absorbance peaks in component B: one at 267 nm and the other at 233 nm. It was concluded that component B contained a nucleotide moiety.
The function of component B was not determined. Cyanocobalamin was found to greatly stimulate methylreductase activity in the component B assay system, but the cause of the stimulation was not determined.
A defined medium was developed for Methanomicrobium mobile; this made possible the study of mobile factor, which is required for the growth of M. mobile. M. mobile was found to require acetate, isobutyrate, 2-methylbutyrate, isovalerate, tryptophan, para-aminobenzoic acid, pyridoxine, thiamine, biotin, and vitamin B(,12) for its growth. Sources of the branched-chain amino acids would not substitute for the branched-chain volatile fatty acids. Indole would substitute for tryptophan, but skatole, anthranilic acid, shikimic acid, or 3-indolelactic acid could not substitute. Folic acid could not substitute for para-aminobenzoic acid. Mobile factor was partially purified by anaerobic chromatography on a Sephadex DEAE-A25 column and a Dowex 50W-X4 column.
Issue Date:1982
Type:Text
Description:106 p.
Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 1982.
URI:http://hdl.handle.net/2142/71156
Other Identifier(s):(UMI)AAI8303004
Date Available in IDEALS:2014-12-16
Date Deposited:1982


This item appears in the following Collection(s)

Item Statistics