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Title:Isozyme and RFLP Studies in the Genus Glycine Willd. Subgenus Glycine
Author(s):Menancio, Desiree Ibanez
Doctoral Committee Chair(s):Hymowitz, Theodore
Department / Program:Agronomy
Degree Granting Institution:University of Illinois at Urbana-Champaign
Subject(s):Biology, Molecular
Biology, Botany
Abstract:The genus Glycine Willd. subgenus Glycine consists of 12 wild perennial species which are native to parts of Australia, South China and adjacent islands in the west and south Pacific. This thesis investigated the variation and biosystematic patterns in the subgenus Glycine utilizing isozyme and RFLP analyses. Three studies were conducted and their results are described below.
First, isozyme variation among 90 accessions representing nine diploid species was analyzed by UPGMA cluster analysis. Ten defined clusters were revealed which were in agreement with classification based on morphology and cytogenetic evidence. The isozyme clusters showed high levels of between accession variation and differentiation among the accessions used. The isozyme evidence provided a good fit to the previously suggested evolutionary lines of development in the subgenus Glycine.
Second, isozyme analysis of 67 accessions of Glycine tabacina (Labill.) Benth. differentiated between diploid and tetraploid cytotypes in the species. Among tetraploid accessions, the isozyme banding patterns observed supported the claim that G. tabacina is an allopolyploid complex.
Third, total DNA from callus tissues of 28 accessions representing seven wild Glycine species were compared using recombinant genomic DNA probes from soybean and corn. Two of the heterologous probes from soybean detected restriction fragment length polymorphisms among the species used. RFLP analysis supported the previous grouping of G. tabacina, G. latifolia and G. microphylla into B-genome species.
Issue Date:1987
Description:129 p.
Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 1987.
Other Identifier(s):(UMI)AAI8803137
Date Available in IDEALS:2014-12-16
Date Deposited:1987

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