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|Title:||The Biology of Eubacterium Limosum and Its Utilization of One-Carbon Compounds|
|Author(s):||Genthner, Barbara Sharak|
|Department / Program:||Dairy Science|
|Degree Granting Institution:||University of Illinois at Urbana-Champaign|
|Abstract:||Eubacterium limosum was isolated as the most numerous methanol-utilizing anaerobe (6.3 x 10('8) cells per ml) in the rumen fluid of two sheep fed a diet in which liquid molasses was a major component.
In addition to energy sources already known, E. limosum grew with methanol, H(,2)-CO(,2), CO, formate, isoleucine or valine as the energy source. Acetate was required for growth with methanol, lactate, valine, and isoleucine, but not with H(,2)-CO(,2). CO(,2) was required for growth with methanol (and H(,2)) and stimulated growth with CO. The CO(,2) requirement could be replaced by formate during growth with methanol. Lipoate, biotin, and pantothenate were the minimal vitamins required for growth with methanol or H(,2)-CO(,2). Cysteine was also required for growth on methanol or H(,2)-CO(,2). Ammonia, some single amino acids, and oligopeptides could serve as the main nitrogen source.
The doubling time for growth with the one-carbon energy sources was 7 h, while the optimum temperature was 39(DEGREES)C and the optimum pH was between 7.0 and 7.2. The cellular yield (g cells/mol substrate utilized) for glucose, methanol, CO, and H(,2) was 70.5, 7.1, 3.4, and 0.84 g, respectively. It grew well in up to 50% CO (2 atm). The K(,m) for methanol and H(,2) was 2.7 x 10('-3) M and 3.4 x 10('-4) M, respectively. The V(,max) for methanol and H(,2) was 1.7 x 10('-6) mols/mg protein(.)min and 1.1 x 10('-7) mols/mg protein(.)min, respectively.
Glucose was preferentially utilized over methanol, H(,2) or isoleucine, but not CO. One-carbon compounds were used simultaneously, except in cultures containing both H(,2) and CO. A substantial amount of CO was utilized before a decrease in H(,2) concentration was observed when both were present.
Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 1984.
|Date Available in IDEALS:||2014-12-16|