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 Title: Effects of Heat and of Ultrasound on Terminally-Differentiated Cells: The Erythrocyte as a Model System Author(s): Kozma, Thomas George Doctoral Committee Chair(s): Ducoff, H.S., Department / Program: Physiology and Biophysics Discipline: Physiology Degree Granting Institution: University of Illinois at Urbana-Champaign Degree: Ph.D. Genre: Dissertation Subject(s): Health Sciences, Radiology Biophysics, Medical Abstract: The effects of heat and of ultrasound were investigated in the terminally- differentiated erythrocyte using hemolysis as end-point. The heat-induced hemolysis of chicken and rat red blood cells (RBC's) was determined between 45 and 51.5$\sp\circ$C. Rat RBC's were more heat sensitive than chicken RBC's. Survival following split-heating was the same as survival following continuous heating when the interval between heatings was 20 min, while survival decreased steadily as this interval was increased to 50 hr.Induction of thermotolerance occurred in chicken RBC's after chronically heating cells at 42.4$\sp\circ$C for 2 hr. Induction of thermotolerance in fresh rat RBC's was not achieved.$\sp{31}$P NMRS was used to monitor changes occurring in the phosphorus metabolites of chicken RBC's during heating. No differences occurred in the levels of nucleoside triphosphates to differentiate heat-shocked RBC's from non-heat-shocked RBC's. The intracellular pH of RBC's changed significantly with temperature, but was the same at a given temperature in both heat-shocked and non-heat-shocked cells.Differences were found between the spectra of heat-shocked and non-heat-shocked RBC's. The spectral line widths of heat-shocked cells were significantly broadened during heat shock and 51.5$\sp\circ$C challenge indicating that hemoglobin became deoxygenated. In addition, a time-dependent left-ward shift of the peak representing the 4,6-P of inositol pentaphosphate occurred during heat shock and remained significantly shifted 0.419 ppm to the left when cells were returned to 23$\sp\circ$C. This suggests that the oxygen-hemoglobin affinity may be reduced in RBC's undergoing a heat shock.Investigations using ultrasound (U.S.) to induce hemolysis showed that damage produced by U.S. could be monitored using the RBC and compared to the damage produced by heat; however, RBC's were not sensitive enough to use for detecting damage at clinically relevant intensities. The rate and kinetics of damage following either modality were significantly different indicating that the two modalities acted on different targets or affected the same targets differently. These results suggest that the mechanism of U.S. damage is different from the effects of other physical agents. Issue Date: 1992 Type: Text Description: 133 p.Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 1992. URI: http://hdl.handle.net/2142/72565 Other Identifier(s): (UMI)AAI9305590 Date Available in IDEALS: 2014-12-17 Date Deposited: 1992
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