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|Title:||Variation and Divergence of Repetitive DNA Sequences With Partial Identity to the Knob Sequence in Tripsacum and Zea|
|Author(s):||Stockton, Tamalyn Kay|
|Doctoral Committee Chair(s):||Hepburn, A.,|
|Department / Program:||Agronomy|
|Degree Granting Institution:||University of Illinois at Urbana-Champaign|
|Abstract:||Sequences with various levels of homology to knob have been isolated from T. dactyloides. These sequences have various degrees of cross-hybridization to knob and are apparently arranged differently in the genome as ascertained by southern blotting. Phylogenetic variation of these sequences indicates that although major features of these repeats have been conserved throughout Tripsacum and Zea, subsets of these repeats have gone through various amplification cycles.
The species specific hybridization pattern of knob DNA in Tripsacum dactyloides indicates that a subset of knob sequences containing an EcoRI site every 180 bp was amplified in this species and to a lesser extent in other Tripsacum species. These EcoRI restriction sites have not previously been reported in knob sequences (Dennis and Peacock, 1984; Viotti et al., 1985).
Another satellite sequence contains a 4.3 kb major HindIII restriction fragment that has been conserved throughout Tripsacum and Zea. However, subsets of this repeat containing differing EcoRI sites have been variously amplified in the taxa examined. Interestingly, the 112bp of this repeat that was sequenced has higher homology (65%) to the radish satellite (Grellet et al., 1986), than to knob (no alignment was able to be made).
The results of this study should be informative on the structure and evolution of the maize genome.
Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 1992.
|Date Available in IDEALS:||2014-12-17|