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ISWS Report of Investigation RI-87PDF

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Title:Development and Evaluation of a Two-Step Membrane Filter Method for Fecal Coliform Recovery in Chlorinated Sewage Effluents
Author(s):Lin, S.D.
Subject(s):fecal coliform
membrane comparison
multiple-tube (MPN) method
two-step membrane filtebacteria analysis
chlorinated wastewater effluents
Duncan's multiple rr method
bacteria analysis
chlorinated wastewater effluents
Duncan's multiple range test
Abstract:A membrane filter (MF) method for fecal coliform (FC) in chlorinated wastewater effluents was developed and evaluated with 10 different types of MFs. A membrane is transferred onto M-FC agar for incubation at 44.5°C for 18±2 hours after pre-enrichment with phenol red lactose broth and incubation at 35°C for 4 hours. Results of 126 comparisons indicated that the FC recoveries by this two-step MF method were comparable to those obtained by the multiple tube (MPN) procedure. The results of comparisons of 10 MF types for FC recovery in chlorinated effluents from five plants with the two-step MF procedure were analyzed by Duncan's multiple range test. The FC recovery rates of the MFs tested can be ranked as follows: Millipore HC ~ Gelman GN-6 ~ Sartorius green > Helena Titan GH ~ Nuclepore N040 ~ Sartorius SM-137 56 ~ Schleicher & Schuell BC 07 > Johns-Manville Sterilized ~ S & S B-9 > J-M Radiation.
Issue Date:1978
Publisher:Illinois State Water Survey
Series/Report:Report of Investigation (Illinois State Water Survey) RI-087
Genre:Technical Report
Type:Text
Language:English
URI:http://hdl.handle.net/2142/77797
Publication Status:published
Peer Reviewed:peer reviewed
Rights Information:Copyright, University of Illinois Board of Trustees. All rights reserved. This document is a product of the Illinois State Water Survey, and has been selected and made available by the Illinois State Water Survey and the University Library, University of Illinois at Urbana-Champaign. It is intended for research and educational use, and proper attribution is requested.
Date Available in IDEALS:2015-05-28


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