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Title:Metabolic Response of Cells Exposed to Magnetomotion of Superparamagnetic Iron Oxide Nanoparticles
Author(s):Flavin, Matthew Thomas
Contributor(s):Boppart, Stephen
Subject(s):cellular metabolism
therapeutics
FLIM
magnetic nanoparticles
magnetomotion
remote-control
Abstract:Magnetomotion of superparamagnetic iron oxide nanoparticles (SPIONs) has recently been studied as a means to provide contrast in image modalities such as ultrasound and optical coherence tomography (OCT). Based on the current knowledge of mechanotransduction within cells, magnetomotion might be capable of inducing a biological effect which could make this technique useful for therapeutic applications. This thesis presents an experimental framework for quantifying the effects of SPION magnetomotion on cellular metabolism as a result of these pathways. Cells were treated by administering SPIONs to subcultures and exposing them to an alternating magnetic field gradient which induced magnetomotion through translational and rotational forces. The metabolic change was assessed using fluorescence lifetime imaging microscopy (FLIM) of endogenous nicotinamide adenine dinucleotide (NADH) molecules whose signal is known to be an indicator of metabolic activity. Evidence in earlier experiments suggests that such treatment is capable of producing an increase in cellular metabolism.
Issue Date:2015-05
Genre:Other
Type:Other
Language:English
URI:http://hdl.handle.net/2142/78986
Date Available in IDEALS:2015-08-03


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