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Title:Chip -Based Detection of Protein Cancer Markers
Author(s):Goluch, Edgar D.
Doctoral Committee Chair(s):Chang Liu
Department / Program:Bioengineering
Discipline:Bioengineering
Degree Granting Institution:University of Illinois at Urbana-Champaign
Degree:Ph.D.
Genre:Dissertation
Subject(s):Biology, Molecular
Abstract:A similar approach was then employed to directly detect protein targets. Instead of DNA, monoclonal antibodies were patterned in the gap. The detector was then exposed to the protein target and gold nanoparticles functionalized with polyclonal antibodies. The target protein was used to immobilize the nanoparticle probes and silver staining was once again employed to bridge the gap It was determined that this technique can be used to detect protein targets both in buffer and serum samples with a 100 pM sensitivity limit.
Issue Date:2007
Type:Text
Language:English
Description:127 p.
Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 2007.
URI:http://hdl.handle.net/2142/79577
Other Identifier(s):(MiAaPQ)AAI3301266
Date Available in IDEALS:2015-09-25
Date Deposited:2007


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