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Title:Lighting Up Life: Fluorescence Studies of the Shaker Potassium Ion Channel and Myosin v in Action
Author(s):Snyder, Gregory Ernst
Doctoral Committee Chair(s):Selvin, Paul R.
Department / Program:Physics
Discipline:Physics
Degree Granting Institution:University of Illinois at Urbana-Champaign
Degree:Ph.D.
Genre:Dissertation
Subject(s):Biophysics, General
Abstract:The other protein I studied is myosin V which walks bipedally along actin filaments taking steps which displace the center of mass 37 nm per step. A controversy over the nature of this movement was settled earlier this year by other members of our laboratory when they showed that myosin V processes in a hand-over-hand manner, with each of the heads alternating in the leading and trailing positions. To do this, they developed a new technique which they called FIONA for Fluorescence Imaging with One Nanometer Accuracy which allowed them to localize individual molecules of myosin V to within 1.5 nut on the surface of a microscope cover slip. I have extended and applied FIONA to a mutant of myosin V with a molecule of the enhanced Green Fluorescent Protein (eGFP) fused to one head of the dieter. I demonstrated that eGFP is suitable to use with FIONA, confirm the hand-over-hand model of myosin procession, and argue that in aggregate, the data on myosin's steps from our lab implies that myosin V adopts an asymmetric, "telemark skier," configuration between steps. (Abstract shortened by UMI.).
Issue Date:2003
Type:Text
Language:English
Description:91 p.
Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 2003.
URI:http://hdl.handle.net/2142/80497
Other Identifier(s):(MiAaPQ)AAI3101972
Date Available in IDEALS:2015-09-25
Date Deposited:2003


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