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Title:Applications of Pump-Probe and Two-Photon Techniques in Time-Resolved Fluorescence Microscopy and Spectroscopy
Author(s):Dong, Chen-Yuan
Doctoral Committee Chair(s):Gratton, Enrico
Department / Program:Physics
Discipline:Physics
Degree Granting Institution:University of Illinois at Urbana-Champaign
Degree:Ph.D.
Genre:Dissertation
Subject(s):Physics, Condensed Matter
Abstract:Two-photon fluorescence microscopy has proven to be an useful technique for imaging biological systems. Good spatial resolution, reduced photobleaching, and efficient rejection of excitation wavelength from emitted fluorescence are the main characteristics of this technique. As part of this work, extension of two-photon microscopy to time-resolved polarization imaging of probe molecules inside STO cells are shown. Depending on the type of probe molecules and the nature of their interaction with cellular constituents, different degrees of rotational correlation times are obtained.
Issue Date:1998
Type:Text
Language:English
Description:104 p.
Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 1998.
URI:http://hdl.handle.net/2142/80655
Other Identifier(s):(MiAaPQ)AAI9904435
Date Available in IDEALS:2015-09-25
Date Deposited:1998


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