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Title:Engineering of Aniline Dioxygenase for Bioremediation and Industrial Applications
Author(s):Ang, Ee Lui
Doctoral Committee Chair(s):Zhao, Huimin; Jeffrey P. Obbard
Department / Program:Chemical Engineering
Discipline:Chemical Engineering
Degree Granting Institution:University of Illinois at Urbana-Champaign
Degree:Ph.D.
Genre:Dissertation
Subject(s):Engineering, Chemical
Abstract:Another round of saturation mutagenesis on active site residues with 1-K31 as parent followed by random mutagenesis yielded the mutant 3-R21. Whole cell activity of 3-R21 for AN, 24DMA and 2IPA were 27.7, 9.8, and 12 nmol/min/mg protein respectively. The activities of 3-R21 for AN, 24DMA and 2IPA were improved by 8.9, 98.0, and 2.0-fold respectively over its parent 1-K31. In particular, the activity of the final mutant 3-R21 was improved by 3.5-fold over the WT AtdA enzyme. Overall, mutant 3-R21 had three mutations---V205A, 1248L, and S404C.
Issue Date:2007
Type:Text
Language:English
Description:202 p.
Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 2007.
URI:http://hdl.handle.net/2142/82405
Other Identifier(s):(MiAaPQ)AAI3301098
Date Available in IDEALS:2015-09-25
Date Deposited:2007


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