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|---|---|---|
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application/pdf  3301098.pdf (5MB) | (no description provided) |
Description
| Title: | Engineering of Aniline Dioxygenase for Bioremediation and Industrial Applications |
| Author(s): | Ang, Ee Lui |
| Doctoral Committee Chair(s): | Zhao, Huimin; Jeffrey P. Obbard |
| Department / Program: | Chemical Engineering |
| Discipline: | Chemical Engineering |
| Degree Granting Institution: | University of Illinois at Urbana-Champaign |
| Degree: | Ph.D. |
| Genre: | Dissertation |
| Subject(s): | Engineering, Chemical |
| Abstract: | Another round of saturation mutagenesis on active site residues with 1-K31 as parent followed by random mutagenesis yielded the mutant 3-R21. Whole cell activity of 3-R21 for AN, 24DMA and 2IPA were 27.7, 9.8, and 12 nmol/min/mg protein respectively. The activities of 3-R21 for AN, 24DMA and 2IPA were improved by 8.9, 98.0, and 2.0-fold respectively over its parent 1-K31. In particular, the activity of the final mutant 3-R21 was improved by 3.5-fold over the WT AtdA enzyme. Overall, mutant 3-R21 had three mutations---V205A, 1248L, and S404C. |
| Issue Date: | 2007 |
| Type: | Text |
| Language: | English |
| Description: | 202 p. Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 2007. |
| URI: | http://hdl.handle.net/2142/82405 |
| Other Identifier(s): | (MiAaPQ)AAI3301098 |
| Date Available in IDEALS: | 2015-09-25 |
| Date Deposited: | 2007 |
This item appears in the following Collection(s)
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Dissertations and Theses - Chemical and Biomolecular Engineering
Dissertations and Theses - Chemical and Biomolecular Engineering -
Graduate Dissertations and Theses at Illinois
Graduate Theses and Dissertations at Illinois