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Title:Elucidation of Protein -Precipitant Phase Diagrams and Their Link to Crystal Quality
Author(s):Talreja, Sameer
Doctoral Committee Chair(s):Kenis, Paul J.A.
Department / Program:Chemical Engineering
Discipline:Chemical Engineering
Degree Granting Institution:University of Illinois at Urbana-Champaign
Degree:Ph.D.
Genre:Dissertation
Subject(s):Engineering, Chemical
Abstract:In sum, we report new evaporation- and dilution-based protocols that will enable structural biologist to rapidly determine the phase diagram (e.g. solubility boundary, metastable zone width) of proteins of unknown structure using a very small sample volume. The knowledge of phase diagram of a protein/precipitant system thus obtained will be useful in obtaining high quality crystals for X-ray diffraction studies. Moreover, we use theory to compare different protein molecules on a generalized phase diagram using the solubility data obtained from our experiments. The comparison of the solubility boundary and the metastable boundary on the same footing will provide a reasonable estimate of the metastable zone width, which will aid crystallographers in screening conditions conducive for protein crystallization. We also develop a kinetic model that describes the competition between the rates of supersaturation, crystal nucleation and crystal growth occurring in the regulated-evaporation crystallization process. The knowledge of these rates coupled with the knowledge of the phase diagram of a protein/precipitant system will enable crystallographers to predict a priori the outcome of an experiment performed in an evaporation-based crystallization platform.
Issue Date:2008
Type:Text
Language:English
Description:139 p.
Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 2008.
URI:http://hdl.handle.net/2142/82410
Other Identifier(s):(MiAaPQ)AAI3314911
Date Available in IDEALS:2015-09-25
Date Deposited:2008


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