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Title:Applications of Traction Force Microscopy in Measuring Adhesion Molecule Dependent Cell Contractility
Author(s):Mann, Cynthia Marie
Doctoral Committee Chair(s):Leckband, D.E.
Department / Program:Chemical Engineering
Discipline:Chemical Engineering
Degree Granting Institution:University of Illinois at Urbana-Champaign
Degree:Ph.D.
Genre:Dissertation
Subject(s):Engineering, Materials Science
Abstract:This work describes the use of polyacrylamide hydrogels as controlled elastic modulus substrates for single cell traction force microscopy studies. The first section describes the use of EDC/NHS chemistry to convalently link microbeads to the hydrogel matrix for the purpose of performing long-term traction force studies (7 days). The final study uses the C2C12 cell line to demonstrate that integrin-mediated adhesion to soft substrates causes different cell behavior than N-cahderin-mediated adhesion to soft substrates. Cells plated on laminin-coated hydrogels exhibited stiffness dependent increases in cell spreading, whereas cells plated on N-cadherin-coated substrates. Similarly, cells plated on laminin-coated substrates exhibited substrate stiffness dependent increases in normalized net contractile moment, however the same cells plated on N-cadherin-coated substrates were unable to deform any but the softest hydrogels.
Issue Date:2009
Type:Text
Language:English
Description:85 p.
Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 2009.
URI:http://hdl.handle.net/2142/82432
Other Identifier(s):(MiAaPQ)AAI3399020
Date Available in IDEALS:2015-09-25
Date Deposited:2009


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