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Title:Molecular Engineering of a Single-Chain FV Antibody Fragment to Femtomolar Affinity
Author(s):Boder, Eric Thomas
Doctoral Committee Chair(s):Wittrup, K. Dane
Department / Program:Chemical Engineering
Discipline:Chemical Engineering
Degree Granting Institution:University of Illinois at Urbana-Champaign
Degree:Ph.D.
Genre:Dissertation
Subject(s):Biology, Molecular
Abstract:Mutants of anti-fluorescein scFv antibody 4-4-20 improved more than 1000-fold with respect to ligand dissociation kinetics and equilibrium binding affinity have been isolated from mutagenized libraries displayed on yeast and screened by flow cytometry. Analysis of the nature of mutations conferring binding improvements suggests a potentially general strategy for engineering scFv antibodies for high affinity binding to target ligands. The results of these studies clearly demonstrate the robustness of the yeast surface display system for quantitatively optimizing recognition parameters of biological macromolecules.
Issue Date:1999
Type:Text
Language:English
Description:148 p.
Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 1999.
URI:http://hdl.handle.net/2142/82463
Other Identifier(s):(MiAaPQ)AAI9921662
Date Available in IDEALS:2015-09-25
Date Deposited:1999


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