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Title:The Production of Recombinant Immunoglobulin Superfamily Proteins in the Yeast Saccharomyces Cerevisiae
Author(s):Shusta, Eric Victor
Doctoral Committee Chair(s):Wittrup, K. Dane
Department / Program:Chemical Engineering
Discipline:Chemical Engineering
Degree Granting Institution:University of Illinois at Urbana-Champaign
Subject(s):Biology, Molecular
Abstract:Although scFvs and scTCRs are structurally homologous, they have very distinct expression and stability behaviors. In fact, in order to develop an efficient scTCR production strategy like that developed for scFvs, it was necessary to alter the characteristics of the protein itself to improve its processing and stability characteristics. These properties were improved using a directed evolution approach and yeast surface display. Evolution resulted in a super-scTCR scaffold with optimized secretion, solubility, and stability properties. This scaffold will, for the first time, enable directed evolution of the binding properties of the scTCR for its peptide/MHC ligand. The results described in this thesis illustrate the power of combining genetic, cellular, and protein engineering tools for improving the secretion and stability characteristics of a target protein.
Issue Date:1999
Description:135 p.
Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 1999.
Other Identifier(s):(MiAaPQ)AAI9953140
Date Available in IDEALS:2015-09-25
Date Deposited:1999

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