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Title:Mechanisms of Asymmetric Protein Localization in Drosophila Embryonic Neural Stem Cells
Author(s):Peng, Chian-Yu
Doctoral Committee Chair(s):Chris Doe
Department / Program:Neuroscience
Degree Granting Institution:University of Illinois at Urbana-Champaign
Subject(s):Biology, Neuroscience
Abstract:By screening a collection of deficiency lines and testing candidate gene involved in cell polarity, we identified lethal giant larvae (lgl) and discs large (dlg) to play key roles in the basal targeting of Prospero and Miranda. In embryonic and larval neuroblasts of lgl and dlg mutants, all basal targeted proteins become uniformly localized but distributions of the apical proteins are unaffected. In neuroblasts, Lgl protein is uniformly distributed at the cell cortex, and its cortical attachment is dependent on Dlg's apically enriched localization. Pharmacological studies suggest Lgl mediated basal targeting occur via a myosin-based transport mechanism, which is supported by genetic studies that show loss of Myosin II suppresses Lgl phenotype in neuroblasts. Taken together, we conclude that Lgl may function to suppress Myosin II activity and allow/promote positive Myosin dependent asymmetric localization in neuroblasts.
Issue Date:2001
Description:166 p.
Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 2001.
Other Identifier(s):(MiAaPQ)AAI3017180
Date Available in IDEALS:2015-09-25
Date Deposited:2001

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