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Title:Identification and Characterization of the Neurotrophic Properties of Dopamine -Releasing Protein (Darp)
Author(s):Smith, Sean Michael
Doctoral Committee Chair(s):Ramirez, Victor D.
Department / Program:Neuroscience
Degree Granting Institution:University of Illinois at Urbana-Champaign
Subject(s):Biology, Neuroscience
Abstract:In a third set of experiments, I assessed the effects of DARP-36aa on the survival of mesencephalic neurons in vitro. Treatment of mesencephalic cultures with DARP36aa resulted in a significant increase in neuron survival and morphological development. Mesencephalic cultures were also incubated with antibodies against DARP-36aa (antiDARP-36aa) in order to assess the effect of immunoneutralization of endogenous DARP on these cells. Mesencephalic cultures treated with anti-DARP-36aa contained significantly fewer neurons, and the number of branching points per neuron was markedly decreased compared to controls. DARP-36aa treatments significantly increased the number of catecholaminergic neurons in mesencephalic cultures while immunoneutralization of endogenous DARP significantly decreased catecholaminergic neuron number and morphological development. Importantly, DARP-36aa-mediated increases in neuron survival and development were completely attenuated by coincubation with genistein. In conclusion, findings from the aforementioned studies suggest that DARP-36aa is a novel neurotrophic factor that promotes survival and development of mesencephalic neurons.
Issue Date:2002
Description:186 p.
Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 2002.
Other Identifier(s):(MiAaPQ)AAI3070441
Date Available in IDEALS:2015-09-25
Date Deposited:2002

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