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Title:The Regulation and Mechanism of Myelin Synthesis Involving Endogenous Steroid Hormones
Author(s):Chan, Jonah R.
Doctoral Committee Chair(s):Glaser, Michael
Department / Program:Neuroscience
Discipline:Neuroscience
Degree Granting Institution:University of Illinois at Urbana-Champaign
Degree:Ph.D.
Genre:Dissertation
Subject(s):Chemistry, Biochemistry
Abstract:In conjunction to the previous study, the platelet-derived growth factor (PDGF) A-chain was detected as two distinct splice variants in Schwann cell/neuronal co-cultures. Both PDGF A-chain isoforms were characterized and examined throughout the myelination process. The mRNA for the long form PDGF A-chain was found to be induced during myelin synthesis and was localized to the DRG neurons. Using an antibody generated against the exon 6 peptide, observations suggest that the splice variant is localized to the surface of axons and to certain Schwann cells that are elongated and ensheathing axons. Since the long form PDGF A-chain is thought to be retained on cellular surfaces by the binding of the basic carboxy-terminal to membrane associated heparan sulfate proteoglycans (HSPG), surface competition experiments were performed with heparin and the basic peptide encoded by exon 6. Heparin and the exon 6 peptide were both found to dramatically inhibit the initiation of myelin formation. While these results are still preliminary, they seem to suggest that the splice variant of the PDGF A-chain/HSPG complex functions as a novel neuronal signal that may regulate steroid biosynthesis in Schwann cells and ultimately lead to the initiation of myelin synthesis.
Issue Date:2000
Type:Text
Language:English
Description:129 p.
Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 2000.
URI:http://hdl.handle.net/2142/82541
Other Identifier(s):(MiAaPQ)AAI9971042
Date Available in IDEALS:2015-09-25
Date Deposited:2000


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