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Title:Structural Study of Natural and Synthetic Polymers
Author(s):Wang, Jinfeng
Doctoral Committee Chair(s):Geil, Phillip H.
Department / Program:Materials Science and Engineering
Discipline:Materials Science and Engineering
Degree Granting Institution:University of Illinois at Urbana-Champaign
Subject(s):Chemistry, Polymer
Abstract:In the second part, the molecular composition, size and conformation of zein, corn prolamines, was examined with MALDI-MS, TEM, SEC3, SAXS and viscometry. MALDI-MS is demonstrated to be a superior technique to study the molecular weight and composition of zein proteins than conventional SDS-PAGE. The observed large particle size from TEM and SEC3 compared to the predicted value from the molecular weight suggested that zein tends to form oligomeric structures. The denaturation behavior of zero was also studied using various denaturants. The results showed most denaturation agents, such as pH, guanidine hydrochloride and DTT, didn't denature zein. Temperature and SDS may cause some denaturation as suggested from the increase of the intrinsic viscosity; the change of intrinsic viscosity was not sufficient to indicate coil-like conformation.
Issue Date:2004
Description:147 p.
Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 2004.
Other Identifier(s):(MiAaPQ)AAI3160966
Date Available in IDEALS:2015-09-25
Date Deposited:2004

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