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Title:Intestinal Inflammatory Responses to the Indigenous Microbiota
Author(s):McCracken, Vance J.
Doctoral Committee Chair(s):Gaskins, H. Rex
Department / Program:Animal Sciences
Discipline:Animal Sciences
Degree Granting Institution:University of Illinois at Urbana-Champaign
Degree:Ph.D.
Genre:Dissertation
Subject(s):Health Sciences, Immunology
Abstract:The potential for inflammatory cytokines to sensitize intestinal epithelial cells to indigenous bacteria was evaluated in HT-29 cells associated with Lactobacillus plantarum 299v in the presence or absence of tumor necrosis factor-alpha (TNF-alpha). Co-culture of TNF-alpha-stimulated HT-29 cells with L. plantarum 299v increased interleukin-8. (IL-8) mRNA expression above levels observed for cells treated with TNF-alpha alone. The ability of TNF-alpha-to upregulate CD14 and Toll-like receptor (TLR) expression and thereby enhance IL-8 mRNA expression in response to L. plantarum 299v was investigated. Baseline expression of CD14 mRNA and cell-surface CD14 was not altered by TNF-alpha. HT-29 cells did not express TLR2 mRNA. However TNF-alpha increased baseline HT-29 expression of TLR4 mRNA, and co-culture with L. plantarum 299v further enhanced TLR4 mRNA expression in TNF-alpha-treated HT-29 cells. These results indicate that neither CD 14 nor TLRs 2 and 4 are involved in the IL-8 mRNA response by HT-29 cells to L. plantarum 299v. However, these results indicate that intestinal epithelial cell responses to indigenous bacteria may contribute to intestinal inflammation.
Issue Date:2001
Type:Text
Language:English
Description:209 p.
Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 2001.
URI:http://hdl.handle.net/2142/83533
Other Identifier(s):(MiAaPQ)AAI3017162
Date Available in IDEALS:2015-09-25
Date Deposited:2001


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