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Title:Biochemical and Genetic Studies on Origin Recognition and DNA Synthesis From the Mesophilic Archaeon Methanosarcina Acetivorans
Author(s):Lin, Li-Jung
Doctoral Committee Chair(s):Isaac Cann
Department / Program:Animal Sciences
Discipline:Animal Sciences
Degree Granting Institution:University of Illinois at Urbana-Champaign
Subject(s):Biology, Microbiology
Abstract:In our study, we characterized DinB and found that DinB was able to synthesize through abasic DNA and cyclobutane pyrimidine dimers, and these activities were stimulated by the cognate PCNA. Fascinatingly, unlike most translesion DNA polymerases previously described, MacDinB synthesized an unusually long product (∼7.2 kb) in the presence of the sliding clamp. A PCNA-interacting peptide (PIP) box located at the C-terminus of MacDinB was shown to facilitate interaction with the sliding clamp. Furthermore, individual mutations at different positions in the PIP-box led to identification of residues that are critical to PCNA/DinB interactions. Nevertheless, the biochemical analysis described here for DinB provides important insights into the properties, potential, and diversity of the Y-family of DNA polymerases.
Issue Date:2009
Description:162 p.
Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 2009.
Other Identifier(s):(MiAaPQ)AAI3392194
Date Available in IDEALS:2015-09-25
Date Deposited:2009

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