Files in this item



application/pdf3017260.pdf (7MB)Restricted to U of Illinois
(no description provided)PDF


Title:Part I. The Synthesis of Hyaluronan Oligosaccharides and the Mild Cleavage of 2-Amino-2-Deoxy-D-Glucoside Methoxycarbonyl Derivatives With Methytrichlorosilane. Part II. The Design and Engineering of a Manganese Binding Site in Cytochrome C Peroxidase
Author(s):Yeung, Bryan K.S.
Doctoral Committee Chair(s):Petillo, Peter A.
Department / Program:Chemistry
Degree Granting Institution:University of Illinois at Urbana-Champaign
Subject(s):Chemistry, Biochemistry
Abstract:Part II. A manganese binding site has been creating in CcP corresponding to the Mn-binding domain in MnP by site directed mutagenesis. Several spectroscopic techniques including paramagnetic NMR and EPR were employed to characterize the CcP mutant (MnCcP). The data is consistent with a newly formed metal site that binds a single Mn2+ near the heme periphery. Wild-type CcP exhibits none of the spectroscopic features as MnCcP. The new binding site provides a five-fold increase in Mn-oxidizing activity in MnCcP over that of WTCcP.
Issue Date:2001
Description:153 p.
Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 2001.
Other Identifier(s):(MiAaPQ)AAI3017260
Date Available in IDEALS:2015-09-25
Date Deposited:2001

This item appears in the following Collection(s)

Item Statistics