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Description
Title: | In Vitro Selection and Characterization of Transition Metal -Dependent DNAzymes and RNAzymes |
Author(s): | Bruesehoff, Peter John |
Doctoral Committee Chair(s): | Lu, Yi |
Department / Program: | Chemistry |
Discipline: | Chemistry |
Degree Granting Institution: | University of Illinois at Urbana-Champaign |
Degree: | Ph.D. |
Genre: | Dissertation |
Subject(s): | Chemistry, Biochemistry |
Abstract: | These DNAzymes are dependent on Co2+ for cleavage activity. By exploiting the electronic properties of Co2+, we have used UV-Visible spectroscopy to obtain a better understanding of the metal binding properties of the selected DNAzyme systems. Through analysis of the spectroscopic and metal binding properties of these systems, in conjunction with the activity properties, a better understanding of how metals are binding in these systems has been developed. A previously selected DNAzyme system, the "8-17" motif, and a naturally-occurring RNAzyme system, the hammerhead motif, were also further examined in terms of the diversity of conditions under which RNA cleavage activity is supported. |
Issue Date: | 2003 |
Type: | Text |
Language: | English |
Description: | 167 p. Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 2003. |
URI: | http://hdl.handle.net/2142/84113 |
Other Identifier(s): | (MiAaPQ)AAI3101806 |
Date Available in IDEALS: | 2015-09-25 |
Date Deposited: | 2003 |
This item appears in the following Collection(s)
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Dissertations and Theses - Chemistry
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Graduate Dissertations and Theses at Illinois
Graduate Theses and Dissertations at Illinois