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Title:In Vitro Selection and Characterization of Transition Metal -Dependent DNAzymes and RNAzymes
Author(s):Bruesehoff, Peter John
Doctoral Committee Chair(s):Lu, Yi
Department / Program:Chemistry
Discipline:Chemistry
Degree Granting Institution:University of Illinois at Urbana-Champaign
Degree:Ph.D.
Genre:Dissertation
Subject(s):Chemistry, Biochemistry
Abstract:These DNAzymes are dependent on Co2+ for cleavage activity. By exploiting the electronic properties of Co2+, we have used UV-Visible spectroscopy to obtain a better understanding of the metal binding properties of the selected DNAzyme systems. Through analysis of the spectroscopic and metal binding properties of these systems, in conjunction with the activity properties, a better understanding of how metals are binding in these systems has been developed. A previously selected DNAzyme system, the "8-17" motif, and a naturally-occurring RNAzyme system, the hammerhead motif, were also further examined in terms of the diversity of conditions under which RNA cleavage activity is supported.
Issue Date:2003
Type:Text
Language:English
Description:167 p.
Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 2003.
URI:http://hdl.handle.net/2142/84113
Other Identifier(s):(MiAaPQ)AAI3101806
Date Available in IDEALS:2015-09-25
Date Deposited:2003


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