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Title:De Novo Heme Protein Design
Author(s):Wang, Jiangyun
Doctoral Committee Chair(s):Kenneth S. Suslick
Department / Program:Chemistry
Degree Granting Institution:University of Illinois at Urbana-Champaign
Subject(s):Chemistry, Biochemistry
Abstract:We have developed an interesting hypothesis for Olfactory Receptor (OR) mechanism. We are so sensitive to thiols and amines that the most natural way to explain this is that OR is a metalloprotein. We have found a consensus sequence "HXXCE" in the 4--5 loop of ORs, which not only binds strongly to Cu2+ and Zn2+, but also turns alpha helical after metal binding. Since the 4--5 loop is as hydrophobic as the fourth helix of OR, charge neutralization of metal ion increases its hydrophobicity, thus it might turn into transmembrane helix and replace the fourth helix. Endogenous ligand binding to the metal site might disturb the charge balance and triggers helix motion, which results in a cell signaling cascade, and eventually the sense of smell.
Issue Date:2003
Description:191 p.
Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 2003.
Other Identifier(s):(MiAaPQ)AAI3101990
Date Available in IDEALS:2015-09-25
Date Deposited:2003

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