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Title:Direct Analysis of Intact Proteins From Microorganisms Using Top Down Mass Spectrometry
Author(s):Meng, Fanyu
Doctoral Committee Chair(s):Kelleher, Neil L.
Department / Program:Chemistry
Discipline:Chemistry
Degree Granting Institution:University of Illinois at Urbana-Champaign
Degree:Ph.D.
Genre:Dissertation
Subject(s):Chemistry, Analytical
Abstract:We applied this top down proteomics platform to study proteins from Methanococcus jannaschii and Saccharomyces cerevisiae. From the whole cell lysate of yeast, ∼120 ion species were successfully identified with 100% sequence coverage, and we also detected several post-translational modifications, including acetylations, phosphorylations, methylations, formylations and oxidations.
Issue Date:2003
Type:Text
Language:English
Description:87 p.
Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 2003.
URI:http://hdl.handle.net/2142/84127
Other Identifier(s):(MiAaPQ)AAI3111620
Date Available in IDEALS:2015-09-25
Date Deposited:2003


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