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Title:Development of High Pressure Surface Plasmon Resonance for the Study of Biosystems
Author(s):Kelley, Jennifer Gann
Doctoral Committee Chair(s):Sligar, Stephen G.
Department / Program:Chemistry
Degree Granting Institution:University of Illinois at Urbana-Champaign
Subject(s):Chemistry, Analytical
Abstract:Through an initial set of experiments high pressure SPR (HP-SPR) was used to measure changes in the reflectivity of various liquids under hydrostatic pressures ranging from ambient to 276 bar (4000 psi). The changes in refractive index as a function of increasing pressure for these solutions were compared and an indication of how these methods can be used to determine volume changes in biomolecular interactions is provided. The application of HP-SPR was extended to the use of commercially available sensor chips. We have shown that BIAcore(TM) SA chips maintain their surface binding functionality after pressure exposure and that effects to the surface are reversible to 6000 psi. Three test systems were chosen to be studied using this instrument, each having different characteristics; cytochrome c association to cytochrome b5, unfolding of staphylococcal nuclease, and binding of the BamH1 restriction enzyme to its DNA recognition site.
Issue Date:2004
Description:163 p.
Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 2004.
Other Identifier(s):(MiAaPQ)AAI3153349
Date Available in IDEALS:2015-09-25
Date Deposited:2004

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