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Description
Title: | Mass Spectrometry and Thiotemplate Assembly: Unique Insights Into the Production of Natural Products on >100 kDa Enzymes |
Author(s): | McLoughlin, Shaun Michael |
Doctoral Committee Chair(s): | Kelleher, Neil L. |
Department / Program: | Chemistry |
Discipline: | Chemistry |
Degree Granting Institution: | University of Illinois at Urbana-Champaign |
Degree: | Ph.D. |
Genre: | Dissertation |
Subject(s): | Chemistry, Biochemistry |
Abstract: | This work further extends initial studies into using FTMS to examine NRP/PK synthetases and provides the most detailed insights into pyochelin, yersiniabactin and FK520 biosynthesis achieved to date. This study also represents the first observation of multiple covalently modified active site peptides in parallel using robust digestion protocols. It is hoped that further refinement of this mass spectrometry-based platform will bolster NRP/PK reengineering efforts towards the design of "unnatural" natural products that combat modern antibiotic-resistant pathogens. |
Issue Date: | 2005 |
Type: | Text |
Language: | English |
Description: | 190 p. Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 2005. |
URI: | http://hdl.handle.net/2142/84195 |
Other Identifier(s): | (MiAaPQ)AAI3199084 |
Date Available in IDEALS: | 2015-09-25 |
Date Deposited: | 2005 |
This item appears in the following Collection(s)
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Dissertations and Theses - Chemistry
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Graduate Dissertations and Theses at Illinois
Graduate Theses and Dissertations at Illinois