Mass Spectrometry and Thiotemplate Assembly: Unique Insights Into the Production of Natural Products on >100 kDa Enzymes

McLoughlin, Shaun Michael

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Title:	Mass Spectrometry and Thiotemplate Assembly: Unique Insights Into the Production of Natural Products on >100 kDa Enzymes
Author(s):	McLoughlin, Shaun Michael
Doctoral Committee Chair(s):	Kelleher, Neil L.
Department / Program:	Chemistry
Discipline:	Chemistry
Degree Granting Institution:	University of Illinois at Urbana-Champaign
Degree:	Ph.D.
Genre:	Dissertation
Subject(s):	Chemistry, Biochemistry
Abstract:	This work further extends initial studies into using FTMS to examine NRP/PK synthetases and provides the most detailed insights into pyochelin, yersiniabactin and FK520 biosynthesis achieved to date. This study also represents the first observation of multiple covalently modified active site peptides in parallel using robust digestion protocols. It is hoped that further refinement of this mass spectrometry-based platform will bolster NRP/PK reengineering efforts towards the design of "unnatural" natural products that combat modern antibiotic-resistant pathogens.
Issue Date:	2005
Type:	Text
Language:	English
Description:	190 p. Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 2005.
URI:	http://hdl.handle.net/2142/84195
Other Identifier(s):	(MiAaPQ)AAI3199084
Date Available in IDEALS:	2015-09-25
Date Deposited:	2005