Files in this item



application/pdf3202106.pdf (4MB)Restricted to U of Illinois
(no description provided)PDF


Title:Investigating Ligand Induced Conformational Changes in the Estrogen Receptor
Author(s):Hurth, Kyle Marran
Doctoral Committee Chair(s):Katzenellenbogen, John A.
Department / Program:Chemistry
Degree Granting Institution:University of Illinois at Urbana-Champaign
Subject(s):Biophysics, General
Abstract:Primarily, we have investigated ER conformational structure using the reporter group method of site-directed spin labeling (SDSL), along with electron paramagnetic resonance (EPR) spectroscopy. In this work, B and C parameter calculations from EPR line-shape theory express receptor differences quantitatively as a spectrum of conformational change. Relative squared difference (RSD) analysis allows us to compare ligand receptor complexes to one another. Furthermore, a rigorous statistical analysis shows that these measurements are reproducible. Placed in context, our work helps further the development of SDSL for the examination of protein conformation, and adds significantly to the hypothesis that the ER exists in a series of dynamic conformational forms depending upon the identity of bound ligand.
Issue Date:2005
Description:169 p.
Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 2005.
Other Identifier(s):(MiAaPQ)AAI3202106
Date Available in IDEALS:2015-09-25
Date Deposited:2005

This item appears in the following Collection(s)

Item Statistics