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Title:Investigating Ligand Induced Conformational Changes in the Estrogen Receptor
Author(s):Hurth, Kyle Marran
Doctoral Committee Chair(s):Katzenellenbogen, John A.
Department / Program:Chemistry
Discipline:Chemistry
Degree Granting Institution:University of Illinois at Urbana-Champaign
Degree:Ph.D.
Genre:Dissertation
Subject(s):Biophysics, General
Abstract:Primarily, we have investigated ER conformational structure using the reporter group method of site-directed spin labeling (SDSL), along with electron paramagnetic resonance (EPR) spectroscopy. In this work, B and C parameter calculations from EPR line-shape theory express receptor differences quantitatively as a spectrum of conformational change. Relative squared difference (RSD) analysis allows us to compare ligand receptor complexes to one another. Furthermore, a rigorous statistical analysis shows that these measurements are reproducible. Placed in context, our work helps further the development of SDSL for the examination of protein conformation, and adds significantly to the hypothesis that the ER exists in a series of dynamic conformational forms depending upon the identity of bound ligand.
Issue Date:2005
Type:Text
Language:English
Description:169 p.
Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 2005.
URI:http://hdl.handle.net/2142/84211
Other Identifier(s):(MiAaPQ)AAI3202106
Date Available in IDEALS:2015-09-25
Date Deposited:2005


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