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Title:Structure and Function of Colicin E5 Ribonuclease Domain
Author(s):Lin, Yi-Lun
Doctoral Committee Chair(s):Raven Huang
Department / Program:Chemistry
Discipline:Chemistry
Degree Granting Institution:University of Illinois at Urbana-Champaign
Degree:Ph.D.
Genre:Dissertation
Subject(s):Biology, Molecular
Abstract:The binding of ImmE5 to ColE5 resulted in a 50% increase of fluorescence emissions. The isothermal titration calorimetry studies showed a -10 kcal/mol enthalpy change upon the formation of ColE5•ImmE5 complex. The mutational studies of ImmE5 indicated that ImmE5 K4 and D95 contributes a larger inhibitory effect toward ColE5. The structure comparison of ColE5 in homodimer and complex showed that ImmE5 not only blocked but also closed the cleft of the active site of ColE5.
Issue Date:2006
Type:Text
Language:English
Description:121 p.
Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 2006.
URI:http://hdl.handle.net/2142/84225
Other Identifier(s):(MiAaPQ)AAI3223658
Date Available in IDEALS:2015-09-25
Date Deposited:2006


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