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Title: | Analysis of D-Aspartate as a Signaling Molecule in the Aplysia Californica Central Nervous System Using Capillary Electrophoresis and Radioisotopic Labeling |
Author(s): | Scanlan, Cory Randolph |
Doctoral Committee Chair(s): | Sweedler, Jonathan V. |
Department / Program: | Chemistry |
Discipline: | Chemistry |
Degree Granting Institution: | University of Illinois at Urbana-Champaign |
Degree: | Ph.D. |
Genre: | Dissertation |
Subject(s): | Chemistry, Analytical |
Abstract: | A second cell-cell signaling molecule investigated is D-aspartate (D-Asp). D-Asp is present in the nervous systems of both vertebrates and invertebrates, and its biosynthesis has been observed in mammalian cells. We suspect that D-Asp acts as a classical neurotransmitter in the central nervous system. The mollusk Aplysia californica has shown high levels of D-Asp in the neural ganglia; and this, along with its relatively simple invertebrate system, makes Aplysia a viable model system for studying the function and neurochemistry of D-Asp. Using Aplysia californica , we have investigated the localization of D-Asp as well as its synthesis from L-Asp. We developed a technique utilizing off-line CE coupled with radionuclide detection, which enables high sensitivity characterization of L- to D-Asp conversion. Furthermore, these capillary electrophoretic techniques, along with liquid scintillation counting and MALDI-MS analysis, have allowed us to address other criteria required to determine whether D-Asp acts as a classical neurotransmitter, including release from cells, sodium dependence of uptake, response to D-Asp by tissues, and the D-Asp stimulated release of cardiomodulatory peptides from the R3-14 cells. These techniques developed provide robust methods for the analysis of neurotransmitters and elucidation of their chiral moieties in complex mass-limited biological samples. |
Issue Date: | 2006 |
Type: | Text |
Language: | English |
Description: | 225 p. Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 2006. |
URI: | http://hdl.handle.net/2142/84251 |
Other Identifier(s): | (MiAaPQ)AAI3250319 |
Date Available in IDEALS: | 2015-09-25 |
Date Deposited: | 2006 |
This item appears in the following Collection(s)
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Dissertations and Theses - Chemistry
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Graduate Dissertations and Theses at Illinois
Graduate Theses and Dissertations at Illinois