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Title:Novel Applications of Top Down MS/MS for Interrogating the Human Proteome
Author(s):Roth, Michael
Doctoral Committee Chair(s):Kelleher, Neil L.
Department / Program:Chemistry
Discipline:Chemistry
Degree Granting Institution:University of Illinois at Urbana-Champaign
Degree:Ph.D.
Genre:Dissertation
Subject(s):Chemistry, Analytical
Abstract:Top Down MS analysis of proteins from human cervical carcinoma cell lines and primary human leukocytes harvested from leukoreduction filters provided identification of 261 protein forms, with ∼12% overlap between the two proteomes. The protein forms were produced by 162 unique genes (∼22% overlap). The above information demonstrates the dynamic expression patterns of proteins in various human cell types. Among the above identifications, 89 proteolysis events, 16 methylation events, 10 phosphorylation sites, 7 acetylations (non-N-terminal), and 9 other modifications were observed. These data indicate the significant post-translational complexity present in the human proteome and validate the application of Top Down in complex proteomes, with the most precise molecular characterization of human proteins demonstrated to date.
Issue Date:2007
Type:Text
Language:English
Description:116 p.
Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 2007.
URI:http://hdl.handle.net/2142/84280
Other Identifier(s):(MiAaPQ)AAI3290364
Date Available in IDEALS:2015-09-25
Date Deposited:2007


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