Files in this item

FilesDescriptionFormat

application/pdf

application/pdf3337728.pdf (5MB)Restricted to U of Illinois
(no description provided)PDF

application/zip

application/zip3337728.zip (28MB)Restricted to U of Illinois
SupplementalZIP

Description

Title:Development and Application of Next Generation Mass Spectrometry for Protein Analysis at High-Resolution
Author(s):Boyne, Michael Thomas, II
Doctoral Committee Chair(s):Kelleher, Neil L.
Department / Program:Chemistry
Discipline:Chemistry
Degree Granting Institution:University of Illinois at Urbana-Champaign
Degree:Ph.D.
Genre:Dissertation
Subject(s):Chemistry, Analytical
Abstract:And finally, a high-resolution MS/MS platform was developed to analyze large peptides at >>50,000 resolving power along with a software suite for interpreting resolved isotopic distributions and handling all known protein modifications on a high throughput basis. Called Middle Down Proteomics, this process identified 7454 peptides from 2-20 kDa (1472 unique) from 555 proteins after just 23 LC-MS/MS injections. Along with greatly increased confidence for peptide identification (expectation values from 10-89 to 104) and characterization (up to 20% of peptides were detected as modified in some LC-MS/MS runs), fragmentation data with <2 ppm accuracy also enabled error tolerant and multiplexed database searching---all clearly demonstrated in this thesis.
Issue Date:2008
Type:Text
Language:English
Description:155 p.
Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 2008.
URI:http://hdl.handle.net/2142/84305
Other Identifier(s):(MiAaPQ)AAI3337728
Date Available in IDEALS:2015-09-25
Date Deposited:2008


This item appears in the following Collection(s)

Item Statistics