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Title:Metal Thiolate and Metal Sufide Clusters in Proteins
Author(s):Savelieff, Masha Georges
Doctoral Committee Chair(s):Lu, Yi
Department / Program:Chemistry
Discipline:Chemistry
Degree Granting Institution:University of Illinois at Urbana-Champaign
Degree:Ph.D.
Genre:Dissertation
Subject(s):Chemistry, Biochemistry
Abstract:Finally efforts towards the design of a biosynthetic model of Cu Z from N2OR are reported. The CuZ cluster is the first and only report to date of a copper-sulfide cluster in Nature. It fulfills a catalytic role in N2OR, performing the reduction of N2O to N2 and H2O during anaerobic respiration, with reducing equivalents provided by a CuA site. The cluster is composed of four copper atoms bridged by a central sulfide, with remaining all histidine coordination to the copper atoms. A scaffold protein was selected, and residues were chosen for mutagenesis to create a similar ligand set to CuZ in N2OR. The copper binding properties and copper/sulfide binding properties of the biosynthetic and native N2OR systems were investigated towards efforts to reconstitute them.
Issue Date:2008
Type:Text
Language:English
Description:232 p.
Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 2008.
URI:http://hdl.handle.net/2142/84319
Other Identifier(s):(MiAaPQ)AAI3337915
Date Available in IDEALS:2015-09-25
Date Deposited:2008


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