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Title:Peptide Analysis Using Capillary Electrophoresis With Fluorescence Detection
Author(s):Oldenburg, Kurt Edward
Doctoral Committee Chair(s):Sweedler, Jonathan V.
Department / Program:Chemistry
Discipline:Chemistry
Degree Granting Institution:University of Illinois at Urbana-Champaign
Degree:Ph.D.
Genre:Dissertation
Subject(s):Chemistry, Biochemistry
Abstract:Since the amino acids tryptophan, tyrosine and phenylalanine contain aromatic functionalities, they can be detected without derivatization when the proper ultraviolet excitation wavelengths are used. The application of wavelength-resolved CE-LIF for the native fluorescence detection of these amino acids and peptides that contain them is described for the classification of peptides based on their spectra. Preliminary results are presented for combining this method with matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS) for the identification of unknown peptides.
Issue Date:1998
Type:Text
Language:English
Description:124 p.
Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 1998.
URI:http://hdl.handle.net/2142/84401
Other Identifier(s):(MiAaPQ)AAI9834722
Date Available in IDEALS:2015-09-25
Date Deposited:1998


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