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Title:Engineering a Purple copper(A) Site Into the Blue Copper Protein Azurin: Construction, Spectroscopy, and Metal Substitution Studies
Author(s):Hay, Michael Todd
Doctoral Committee Chair(s):Lu, Yi
Department / Program:Chemistry
Discipline:Chemistry
Degree Granting Institution:University of Illinois at Urbana-Champaign
Degree:Ph.D.
Genre:Dissertation
Subject(s):Chemistry, Biochemistry
Abstract:Metal substitution studies of azurin-CuA have been performed using various metal ions (i.e., Hg2+, Ag+, Cu +, Cd+, Rh2+, Au+, Co 2+, and Ni2+). Metal binding was determined by electronic absorption and electrospray mass spectrometry. The results obtained from theses studies indicate that there are two factors that may influence binding in azurin-CuA: (1) the site tends to bind metal combinations which produce an overall +3 charge, and (2) the site binds soft thiophilic metals. Further characterization of the above derivatives will contribute to the understanding of the metal-binding affinity, rigidity, and other structural properties of purple copper centers.
Issue Date:1998
Type:Text
Language:English
Description:177 p.
Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 1998.
URI:http://hdl.handle.net/2142/84423
Other Identifier(s):(MiAaPQ)AAI9912257
Date Available in IDEALS:2015-09-25
Date Deposited:1998


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