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Title:In Vitro Selection of Highly Efficient DNA Enzymes as RNA Nucleases and Metal Biosensors
Author(s):Li, Jing
Doctoral Committee Chair(s):Lu, Yi
Department / Program:Chemistry
Discipline:Chemistry
Degree Granting Institution:University of Illinois at Urbana-Champaign
Degree:Ph.D.
Genre:Dissertation
Subject(s):Chemistry, Biochemistry
Abstract:Based on the superior activity of 17E in Pb2+ than in other metal ions, we evaluated its potential application in sensing Pb 2+ ions. Combining the high selectivity of 17E towards Pb2+ and the high sensitivity of fluorescence detection, 17E can measure the presence of Pb2+ ions over a concentration range of three orders of magnitude, with a detection limit as low as 10 nM. This system represents the first example of DNA-based biosensor for metal ions. Similar approaches can be applied to develop deoxyribozymes that can probe other metal ions and at various concentration ranges.
Issue Date:2000
Type:Text
Language:English
Description:153 p.
Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 2000.
URI:http://hdl.handle.net/2142/84486
Other Identifier(s):(MiAaPQ)AAI9990060
Date Available in IDEALS:2015-09-25
Date Deposited:2000


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