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Title:The Membrane Topology of the Microsomal Cytochromes P450: Interfacial Characterization of Protein Structure
Author(s):Retzlaff, Mary Lillian Shank
Doctoral Committee Chair(s):Sligar, Stephen G.
Department / Program:Chemistry
Degree Granting Institution:University of Illinois at Urbana-Champaign
Subject(s):Biology, Molecular
Abstract:In order to characterize this secondary-binding interaction, a novel surface plasmon resonance technique termed "analyte gradient-surface plasmon resonance" (AG-SPR) has been developed and is described here. AG-SPR can be used to rapidly characterize macro-molecular interactions without requiring surface regeneration and has been used to monitor the binding of two truncated P450s with a phosphatidylcholine supported lipid bilayer. The data has been fit to a kinetic model that incorporates a semi-empirical available surface function to account for the effect of steric interactions between pre-adsorbed molecules. The results indicate that P450 does in fact contain a peripheral binding interaction. However, in order to determine an accurate binding affinity, additional factors must be incorporated into the kinetic model.
Issue Date:2000
Description:132 p.
Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 2000.
Other Identifier(s):(MiAaPQ)AAI9990124
Date Available in IDEALS:2015-09-25
Date Deposited:2000

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