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Title:Estrogen Regulation of Proteinase Inhibitor 9 Gene Expression and Interleukin-1beta Production
Author(s):Stobie-Krieg, Sacha Ann Skookum
Doctoral Committee Chair(s):Shapiro, David J.
Department / Program:Biochemistry
Degree Granting Institution:University of Illinois at Urbana-Champaign
Subject(s):Biology, Molecular
Abstract:The ability of several estrogens and antiestrogens to activate PI-9 gene expression was assessed by transient transfection. Of interest, the mixed agonist/antagonist tamoxifen (OHT) was an agonist on the transiently transfected reporter gene but behaved as an antagonist on the cellular gene. To begin to assess how OHT-ER interacts with the native PI-9 promoter we carded out chromatin immunoprecipitation (ChIP) assays. The ChIP assays demonstrated that moxestrol-ER complex bound to the PI-9 promoter, but that OHT-ER (and unliganded ER) did not bind. These data provide the first evidence that the agonist activity of OHT-ER stems from the inability of OHT to elicit conformational changes in chromatin which allow ER to bind to ERE's.
Issue Date:2001
Description:148 p.
Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 2001.
Other Identifier(s):(MiAaPQ)AAI3023204
Date Available in IDEALS:2015-09-25
Date Deposited:2001

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