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Title:Kinetic and Mechanistic Characterization of the Uracil Phosphoribosyltransferase Activity of Bacillus Subtilis PyrR
Author(s):Grabner, Gail Keaton
Doctoral Committee Chair(s):Switzer, Robert L.
Department / Program:Biochemistry
Discipline:Biochemistry
Degree Granting Institution:University of Illinois at Urbana-Champaign
Degree:Ph.D.
Genre:Dissertation
Subject(s):Chemistry, Biochemistry
Abstract:Direct binding of ligands to PyrR was studied by equilibrium dialysis. The dissociation constant calculated for UMP was 27 +/- 3 muM, whereas the KD for PRPP was 18 +/- 2 muM. There was no detectable binding of uracil or PPi to PyrR, which was consistent with each of the four proposed conformational change models.
Issue Date:2002
Type:Text
Language:English
Description:259 p.
Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 2002.
URI:http://hdl.handle.net/2142/84785
Other Identifier(s):(MiAaPQ)AAI3070311
Date Available in IDEALS:2015-09-25
Date Deposited:2002


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