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Title:PaaG and PaaF: New Members of the Crotonase Superfamily
Author(s):Lies, Mark Andrew, III
Doctoral Committee Chair(s):Gerlt, John A.
Department / Program:Biochemistry
Discipline:Biochemistry
Degree Granting Institution:University of Illinois at Urbana-Champaign
Degree:Ph.D.
Genre:Dissertation
Subject(s):Biology, Molecular
Abstract:Using the published operon structure of phenylacetate utilization, BLAST searches of public sequence databases, and metabolic information from a related compound, two pathways for phenylacetate metabolism were proposed. The genes encoding PaaF, PaaG, PaaH, PaaJ, and PaaI were cloned, and the proteins expressed, and purified. I showed by enzyme assays using purified proteins that beta-oxidation in phenylacetate metabolism proceeds via adipyl-CoA intermediates. I showed that PaaG catalyzes the isomerization of trans-Delta 3-hexenedioyl-CoA to trans-Delta2-hexenedioyl-CoA, uses an Asp as a general base, and the reaction proceeds without incorporation of solvent protons.
Issue Date:2003
Type:Text
Language:English
Description:199 p.
Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 2003.
URI:http://hdl.handle.net/2142/84793
Other Identifier(s):(MiAaPQ)AAI3086121
Date Available in IDEALS:2015-09-25
Date Deposited:2003


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