Files in this item



application/pdf3086200.pdf (5MB)Restricted to U of Illinois
(no description provided)PDF


Title:Modulators of Inflammation and Steroid Receptors Regulate Proteinase Inhibitor 9 Gene Expression
Author(s):Thulasiraman, Padmamalini
Doctoral Committee Chair(s):Shapiro, David J.
Department / Program:Biochemistry
Degree Granting Institution:University of Illinois at Urbana-Champaign
Subject(s):Chemistry, Biochemistry
Abstract:In addition to pro-inflammatory agents, the synthetic anti-inflammatory glucocorticoid, dexamethasone, induced PI-9 gene expression via an imperfect glucocorticoid response element (GRE) located at -208. Dexamethasone induced PI-9 mRNA and protein. Dexamethasone-glucocorticoid receptor (GR) complex inhibited IL-1beta induced PI-9 gene expression but IL-1beta did not interfere with dexamethasone induction of PI-9. Glucocorticoid administration resulted in reduced p65 binding to a consensus NF-kappaB element. To investigate the mechanism of crosstalk between Dex-GR and NF-kappaB, we analyzed the effect of dexamethasone on induction of IkappaB-alpha, the inhibitory heterodimer of p65. Dexamethasone induced IkappaBalpha in HepG2 cells. This may be one of the mechanisms used by Dex-GR to interfere with NF-kappaB activity and block IL-1beta-induced PI-9 gene expression. Our findings show that liganded GR may regulate IL-1beta induction of PI-9 expression through NF-kappaB and AP-1 transcription factors by a mechanism quite different than that seen for estrogen-ER.
Issue Date:2003
Description:112 p.
Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 2003.
Other Identifier(s):(MiAaPQ)AAI3086200
Date Available in IDEALS:2015-09-25
Date Deposited:2003

This item appears in the following Collection(s)

Item Statistics