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Title:Structure and Biochemistry of RsrI Methyltransferase
Author(s):Thomas, Chad Baldo
Doctoral Committee Chair(s):Gumport, Richard I.
Department / Program:Biochemistry
Discipline:Biochemistry
Degree Granting Institution:University of Illinois at Urbana-Champaign
Degree:Ph.D.
Genre:Dissertation
Subject(s):Chemistry, Biochemistry
Abstract:Comparison of the dimer interface of M.RsrI with the recent M.MboII structure and sequence alignments allowed identification of a conserved dimerization motif. I disrupted the dimerization of M.RsrI by adding N-acetyl-phenylalanine as a competitive inhibitor and by mutation of a serine located in the dimer interface to an aspartate. Both methods resulted in inhibition of the enzyme activity, suggesting dimerization is important for enzyme activity.
Issue Date:2003
Type:Text
Language:English
Description:125 p.
Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 2003.
URI:http://hdl.handle.net/2142/84801
Other Identifier(s):(MiAaPQ)AAI3101981
Date Available in IDEALS:2015-09-25
Date Deposited:2003


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