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Title:Fatty Acid Degradation in Escherichia Coli and Salmonella Enterica Serovar Typhimurium
Author(s):Iram, Surtaj H.
Doctoral Committee Chair(s):Cronan, John E., Jr
Department / Program:Biochemistry
Discipline:Biochemistry
Degree Granting Institution:University of Illinois at Urbana-Champaign
Degree:Ph.D.
Genre:Dissertation
Subject(s):Biology, Molecular
Abstract:Wild type strains of S. enterica grow on decanoic acid whereas wild type E. coli strains cannot. Mutant strains (fadR) of both organisms are readily isolated in which the genes (fad) of fatty acid degradation are expressed constitutively. The S. enterica fadR strains grow more rapidly than the wild type strains on decanoic acid and also grow well on octanoic and hexanoic acids. In contrast, Escherichia coli fadR strains grow well on decanoic acid, but grow only exceedingly slowly on octanoic acid and fail to grow at all on hexanoic acid. This difference in growth was partly attributed to S. enterica having higher cytosolic levels of the inducing ligand, fatty acyl-CoAs. The most striking results were the differences in the compositions of CoA metabolites of strains grown with octanoic acid or oleic acid. S. enterica cleanly converted all of the acid to acetyl-CoA whereas E. coli accumulated high levels of intermediate chain length products. Exchanging genes between the two organisms showed that the S. enterica FadE and FadBA enzymes were responsible for the greater efficiency of beta-oxidation.
Issue Date:2005
Type:Text
Language:English
Description:113 p.
Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 2005.
URI:http://hdl.handle.net/2142/84817
Other Identifier(s):(MiAaPQ)AAI3199027
Date Available in IDEALS:2015-09-25
Date Deposited:2005


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