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Title:Natural and Engineered Promiscuity Within the Enolase Superfamily
Author(s):Millikin, Cheri Lynn
Doctoral Committee Chair(s):Gerlt, John A.
Department / Program:Biochemistry
Discipline:Biochemistry
Degree Granting Institution:University of Illinois at Urbana-Champaign
Degree:Ph.D.
Genre:Dissertation
Subject(s):Chemistry, Biochemistry
Abstract:The dehydration of both D-mannonate, via syn-elimination, and D-altronate, via anti-elimination, is analogous to the stereochemical promiscuity engineered into the GlucD active site. The redesign of GlucD and the characterization of RspA illustrate the same type of promiscuity: syn and anti dehydrations in the same active site with different substrates, which results from proton abstraction of stereochemically equivalent protons. These experiments illustrate how Nature could have utilized the (beta/alpha)8-barrel as a scaffold to evolve new activities.
Issue Date:2005
Type:Text
Language:English
Description:256 p.
Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 2005.
URI:http://hdl.handle.net/2142/84818
Other Identifier(s):(MiAaPQ)AAI3199089
Date Available in IDEALS:2015-09-25
Date Deposited:2005


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