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Title:A Study of the Mechanistic Diversity Inherent to the Ribulose Phosphate Binding Barrel Superfamily
Author(s):Akana, Julie A.
Doctoral Committee Chair(s):Gerlt, John A.
Department / Program:Biochemistry
Discipline:Biochemistry
Degree Granting Institution:University of Illinois at Urbana-Champaign
Degree:Ph.D.
Genre:Dissertation
Subject(s):Chemistry, Biochemistry
Abstract:To probe the functional plasticity of the RPBB superfamily, rational design was used to enhance the UPS activity within the KGPDC scaffold. The triple mutant E112D/T169A/R139V had an enhancement in kcat /KM by 260 fold over wild-type KGPDC. Structural studies suggest that little perturbation of the active site occurs with the 3 mutations.
Issue Date:2006
Type:Text
Language:English
Description:160 p.
Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 2006.
URI:http://hdl.handle.net/2142/84822
Other Identifier(s):(MiAaPQ)AAI3223532
Date Available in IDEALS:2015-09-25
Date Deposited:2006


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