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Title:Studies on the Proton Pumping Mechanism of Aa(3)-Type Cytochrome C Oxidase
Author(s):Han, Dan
Doctoral Committee Chair(s):Gennis, Robert B.
Department / Program:Biochemistry
Discipline:Biochemistry
Degree Granting Institution:University of Illinois at Urbana-Champaign
Degree:Ph.D.
Genre:Dissertation
Subject(s):Chemistry, Biochemistry
Abstract:The monomeric form of bovine heart mitochondrial cytochrome c oxidase was used to detect directly the proton uptake and release events during the F-to-O transition. Absorption change of the pH sensitive dye was monitored spectroscopically. Rapid proton release followed by slow proton uptake was observed. This result indicates that both the pumped protons and the protons required for chemistry at the active site might come from an internal proton pool containing at least two protons.
Issue Date:2006
Type:Text
Language:English
Description:192 p.
Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 2006.
URI:http://hdl.handle.net/2142/84824
Other Identifier(s):(MiAaPQ)AAI3223606
Date Available in IDEALS:2015-09-25
Date Deposited:2006


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