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Title:Characterization of the Fidelity Mechanisms of Leucyl -Trna Synthetases From Saccharomyces Cerevisiae and Escherichia Coli
Author(s):Karkhanis, Vrajesh Anil
Doctoral Committee Chair(s):Martinis, Susan A.
Department / Program:Biochemistry
Discipline:Biochemistry
Degree Granting Institution:University of Illinois at Urbana-Champaign
Degree:Ph.D.
Genre:Dissertation
Subject(s):Chemistry, Biochemistry
Abstract:We tested several mutations in yeast mitochondrial LeuRS that altered the post-transfer editing function of LeuRSs from other origins. Our results show that yeast mitochondrial LeuRS has maintained a competent editing active site for post-transfer editing of mischarged tRNA similar to other LeuRSs. However, unlike other origins, when LeuRS amino acid editing-defective mutants were introduced in vivo, cell viability was largely unaffected. In contrast, these editing-defective mutations limited viability of E. coli cells. We propose that the yeast mitochondria have evolved to tolerate lower levels of fidelity in protein synthesis.
Issue Date:2007
Type:Text
Language:English
Description:130 p.
Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 2007.
URI:http://hdl.handle.net/2142/84840
Other Identifier(s):(MiAaPQ)AAI3269936
Date Available in IDEALS:2015-09-25
Date Deposited:2007


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